Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Inflammatory cytokines regulate function and expression of adenosine A(2A) receptors in human monocytic THP-1 cells.

doi: 10.4049/jimmunol.167.7.4026

Figure Lengend Snippet: FIGURE 1. Effect of inflammatory cytokines on A2A receptor-mediated production of IL-12 in LPS-activated THP-1 cells. THP-1 cells were pretreated with IL-1 (10 U/ml), TNF- (100 U/ml), IFN- (100 U/ml), or medium alone (Control) and then stimulated with CGS-21680 and LPS (5 g/ml) as described in Materials and Methods. Culture supernatants were collected and subject to ELISA for IL-12. A, Inhibition of IL-12 p70 secretion in LPS-treated cells in response to CGS-21680. The basal levels (without CGS-21680 stimulation) of IL-12 p70 were 29.4 13.3 pg/ml (Control), 26.5 12.4 pg/ml (IL-1), 24.3 13.5 pg/ml (TNF-), and 66.5 27.5 pg/ml (IFN-). B, Inhibition of IL-12 p40 secretion in response to CGS-21680. The basal levels of IL-12 p40 were 237.8 100.0 pg/ml (Control), 201.4 73 pg/ml (IL-1), 136.8 49.3 pg/ml (TNF-), and 724.4 267 pg/ml (IFN-). C, The inhibitory effect of CGS-21680 (CGS) on IL-12 p40 secretion was greatly blocked in the presence of the A2A receptor antagonist ZM-241385 (ZM, 10 M). Shown are representative results of a single experiment of two experiments with similar results. The basal levels of IL-12 p40 in the presence of ZM-241385 were 256.2 pg/ml (Control), 260 pg/ml (IL-1), 196.4 pg/ml (TNF-), and 685.8 pg/ml (IFN-). For A and B, data are presented as mean SEM of three independent experiments.

Article Snippet: Culture supernatants were collected and subjected to assays for human IL-12 p70 (the heterodimer form), IL-12 p40 subunit, and IL-10 using ELISA Quantikine kits purchased from R&D Systems. cAMP assay THP-1 cells were pretreated overnight with the cytokines.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Inhibition

Journal: Cardiovascular Diabetology

Article Title: Osteocalcin attenuates high fat diet-induced impairment of endothelium-dependent relaxation through Akt/eNOS-dependent pathway

doi: 10.1186/1475-2840-13-74

Figure Lengend Snippet: Serum levels of atherogenic inflammatory factors decreased in OCN-treated compared with vehicle-treated ApoE-KO mice. (A) TNF-α (B) IL-1α (C) IL-12 p70 (D) IL-12 p40. Data were shown as mean ± SEM, 6 to 8 mice per group were analyzed. ** P < 0.01 vehicle-treated mice fed with HFD vs. vehicle-treated mice fed with chow diet; # P < 0.05, ## P < 0.01 OCN-treated mice fed with HFD vs. vehicle-treated mice fed with HFD. HF, high fat diet; CD, chow diet; OCN, osteocalcin.

Article Snippet: TNF-α, IL-1α, IL-12 p70 and IL-12 p40 ELISA kits were purchased from R&D Systems (Minneapolis, Minnesota, USA).

Techniques: